Optimizing Electrical Penetration Graph Settings to Record Blue-green Sharpshooter X Waves for Future Studies of Grape Host Plant Resistance to Xylella fastidiosa Inoculation
نویسندگان
چکیده
• Suitability of Fertilized and Unfertilized Eggs of the Glassy-winged Sharpshooter for the Egg Parasitoid Gonatocerus morrilli Rodrigo Krugner ............................................................................................................................................. 11 • A Method to Quantify Glassy-winged Sharpshooter Egg Maturation Mark Sisterson ................................................................................................................................................ 12
منابع مشابه
Correlation of stylet activities by the glassy-winged sharpshooter, Homalodisca coagulata (Say), with electrical penetration graph (EPG) waveforms.
Glassy-winged sharpshooter, Homalodisca coagulata (Say), is an efficient vector of Xylella fastidiosa (Xf), the causal bacterium of Pierce's disease, and leaf scorch in almond and oleander. Acquisition and inoculation of Xf occur sometime during the process of stylet penetration into the plant. That process is most rigorously studied via electrical penetration graph (EPG) monitoring of insect f...
متن کاملTransmission of Xylella fastidiosa to grapevines by Homalodisca coagulata (Hemiptera: Cicadellidae).
Pierce's disease (PD) of grapevines is caused by a xylem-limited bacterium Xylella fastidiosa (Wells, Raju, Hung, Weisburg, Mandelco-Paul, and Brenner) that is transmitted to plants by xylem sap-feeding insects. The introduction of the sharpshooter leafhopper Homalodisca coagulata (Say) into California has initiated new PD epidemics in southern California. In laboratory experiments, the major c...
متن کاملCOMPARATIVE PROTEOMIC ANALYSIS OF STEM TISSUE AND XYLEM SAP FROM PIERCE’S DISEASE RESISTANT AND SUSCEPTIBLE GRAPEVINES Project Leaders:
Analyses of host plant resistance / susceptibility mechanisms to Xylella fastidiosa (Xf) infection are critical for understanding host-pathogen interactions. Proteomic analyses of stem tissue and xylem sap samples were initiated to complement genomic approaches employed in elucidating Pierce’s disease resistance mechanisms. Samples from one highly resistant and two susceptible grape genotypes w...
متن کاملStylet Penetration Behaviors of Graphocephala atropunctata (Signoret) (Hemiptera, Cicadellidae): EPG Waveform Characterization and Quantification
Weanalyzed theprobing (stylet penetration)behaviors of the sharpshooter leafhopper Graphocephala atropunctata (Signoret) on grape with an alternating current (AC) electrical penetration graph (EPG) monitor. We characterized waveforms likely to represent stylet penetration pathway phase and xylem ingestion. The total probing duration of the cohort represented 68% of all 20-h monitoring periods f...
متن کاملIMPACT OF MULTIPLE-STRAIN INFECTIONS OF XYLELLA FASTIDIOSA ON ACQUISITION AND TRANSMISSION BY THE GLASSY-WINGED SHARPSHOOTER Project Leaders:
In this project, studies were conducted to 1) develop a method to detect and differentiate multiple strains of Xylella fastidiosa in individual glassy-winged sharpshooters (GWSS,) and 2) determine the relative ability of an individual GWSS to simultaneously retain or transmit two strains of X. fastidiosa. Strain-specific primers were developed that can detect and differentiate the Pierce’s dise...
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